Fig. 5: Liquid-to-solid phase transition and amyloid aggregation of αS/Tau441 coacervates. | Nature Communications

Fig. 5: Liquid-to-solid phase transition and amyloid aggregation of αS/Tau441 coacervates.

From: Molecular mechanism for the synchronized electrostatic coacervation and co-aggregation of alpha-synuclein and tau

Fig. 5

a WF microscopy images of 25 μM αS in the presence of 25 μM Tau441 (1 μM AF488-labeled αS and Atto647N-labeled Tau441) in LLPS buffer at different incubation times and focal heights (z, distance from the bottom of the non-binding plate well). Six experiments were repeated independently with similar results. b CF microscopy images of 25 μM αS in the presence of 25 μM Tau441 (1 μM Atto647N-labeled Tau441) and 12.5 μM thioflavin-T (ThT). Suspended protein droplets and deposited protein rafts and puncta are shown in the top and middle rows, respectively. The bottom row shows images of rafts and puncta from 3 independent replicate experiments. White arrowheads indicate ThT-positive puncta in both panels. Scale bar is 20 μm for all images.

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