Fig. 1: COVID19 severity correlates with an increase in blood neutrophil proportion and epigenetic changes in genes related with the innate immune response.
a Methylome deconvoluted blood cell proportions are plotted by cohort (left panel discovery, right panel replication) and group (blue, 47 and 54 negative SARS-CoV2 lab tested individuals for discovery and replication; yellow, 269 and 91 positive individuals with mild symptoms for discovery and replication and red, 98 and 15 positive individuals with severe symptoms for discovery and validation). Paired differences were assessed by means of linear regression analysis (age and sex were included as covariates) and significance values plotted by pairs (.p-value < 0.05, *p-value < 0.01 and **p-value < 1e-5). The center line denotes the median value, the box contains 25th to 75th percentiles of the dataset and the whiskers extend up to ± 1.5*IQR. b Venn diagram with the number of significant shared DMCs across the differential analysis performed (the number of annotated genes are included in parentheses). c Combined manhattan plots are shown for the differential analysis that share DMCs, hypermethylated and hypomethylated DMCs are divided into upper and lower side of the manhattan plot respectively. Genes annotated for the shared DMCs are depicted, including, in parentheses their co-localization with the annotated gene (TSS, Transcription Start Site: Body, gene body). Severe vs negative (blue), mild vs negative (green), severe vs mild (yellow) and pseudotime longitudinal analysis (red).
