Fig. 2: OsOTUB1-cat is likely induced to be active by distal Ub, but may not be activated by proximal Ub. | Nature Communications

Fig. 2: OsOTUB1-cat is likely induced to be active by distal Ub, but may not be activated by proximal Ub.

From: Met1-specific motifs conserved in OTUB subfamily of green plants enable rice OTUB1 to hydrolyse Met1 ubiquitin chains

Fig. 2

a Crystal structure of OsOTUB1-cat; b Structural alignment between OsOTUB1-cat and hOTUB1-cat; c Crystal structure of OsOTUB1-cat~Met1-diUb-DHA and its alignment with OsOTUB1-cat; d Structural alignment between catalytic centers of OsOTUB1-cat and OsOTUB1-cat~Met1-diUb-DHA; e Effects of Q2 mutation in the proximal Ub on Met1-diUb hydrolytic activity (N = 3 biologically independent experiments); f Two replicates’ kinetics data of OsOTUB1-cat against Met1-diUb variants (N = 2 biologically independent experiments, individual data shown as separate values); g Time-scale Ub-AMC cleavage (N = 3 biologically independent experiments); h Time scale Ub-PA crosslinking activity of OsOTUB1-cat and OTULIN-cat (N = 3 biologically independent experiments); i Crystal structure of OsOTUB1-cat~Ub-PA and its alignment with OsOTUB1-cat. Source data are provided as a Source Data file.

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