Fig. 4: Agonist-induced βarr1 recruitment to V2RWT and V2RT360A mutant.
From: Allosteric modulation of GPCR-induced β-arrestin trafficking and signaling by a synthetic intrabody
a Schematic representation of NanoBiT-based βarr1 recruitment assay. b HEK-293 cells expressing the indicated receptor and βarr1 constructs were stimulated with varying doses of AVP for 30 min followed by the measurement of luminescence (mean ± SEM; n = 4 independent experiments; normalized with luminescence signal for V2RWTat maximal ligand dose as 100%, Two-way ANOVA, Sidak’s multiple comparisons test; ****p < 0.0001). c Schematic representation of NanoBiT-based assay for measuring βarr1 translocation to the cell surface. d HEK-293 cells expressing the indicated receptor and βarr1 constructs together with LgBiT-CAAX were stimulated with varying doses of AVP for 30 min followed by the measurement of luminescence (mean ± SEM; n = 4 independent experiments; normalized with luminescence signal for V2RWTat maximal ligand dose as 100%, Two-way ANOVA, Sidak’s multiple comparison test; ns = non-significant). Source data are provided as a Source Data file.
