Fig. 2: P17 tag significantly improved solubility of scFv in SHuffle T7 strain regardless of fusion positions. | Nature Communications

Fig. 2: P17 tag significantly improved solubility of scFv in SHuffle T7 strain regardless of fusion positions.

From: A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments

Fig. 2

a Schematic diagrams of His-MA18/7-scFv-HA protein and its N- and C-terminally P17-tagged derivatives with a C-terminal HA tag fusion or not. b Determination of solubility of His-MA18/7-scFv-HA protein and its derivatives expressed in SHuffle T7 cells by western blotting. The result of one representative experiment was shown. T, total protein; S, soluble protein. c Solubility of His-MA18/7-scFv-HA and its derivatives were determined by three independent experiments. Data are presented as mean values ± SD. The statistical significance of differences between two experimental groups was assessed through one-way analysis of variance (ANOVA). d Fold change of solubility of P17-tagged derivatives of His-MA18/7-scFv-HA to untagged version.

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