Fig. 3: A C-terminal P17 tag increased scFv solubility in SHuffle T7 cells more efficiently than N- or C-terminal MBP and N-terminal GST. | Nature Communications

Fig. 3: A C-terminal P17 tag increased scFv solubility in SHuffle T7 cells more efficiently than N- or C-terminal MBP and N-terminal GST.

From: A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments

Fig. 3

a Determination of solubility of each G12-scFv fusion protein with HA and P17 tag attached at C-terminus, MBP tag at N or C-terminus, or GST tag at N terminus by western blotting. T, total protein; S, soluble protein. b Solubility of G12-scFv fusion proteins was determined by three independent experiments. c Fold change of solubility of P17, MBP, and GST-tagged scFvs compared to HA-tagged scFv. Data are presented as mean values ± SD. The statistical significance of differences between the two experimental groups was assessed by one-way ANOVA.

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