Fig. 4: Effects of fusion of one, two, or three C-terminal P17 tag(s) on scFv’s solubility in SHuffle T7 cells. | Nature Communications

Fig. 4: Effects of fusion of one, two, or three C-terminal P17 tag(s) on scFv’s solubility in SHuffle T7 cells.

From: A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments

Fig. 4

a Determination of solubility of MA18/7-scFv-HA protein and MA18/7-scFv fusion protein with one, two, and three P17 tag(s) individually attached at the C-terminus by western blotting. T total protein, S soluble protein. b Solubility of MA18/7-scFv-HA and three other MA18/7-scFv fusion proteins were determined by three independent experiments. c Fold change of solubility of scFvs fused to different numbers of P17 tags to untagged one. Data are presented as mean values ± SD. The statistical significance of differences between the two experimental groups was assessed by one-way ANOVA.

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