Fig. 4: Gene Expression Dynamics.

a Global gene expression patterns before and after pre-exposure for each phenotype. Color represents phenotype and line type represents timepoint. Note design did not include replication of colony at T2, so no analysis is performed. Timepoints and phenotypes were compared using PERMANOVA. b Relationship between experimental Degree Heating Weeks during pre-exposure and average log₂Fold-Change of all contigs. The gray line represents overall relationship and shaded area is 95% CI of linear regression fit. Color represents treatment. c Mean absolute value of log₂Fold-Change for all contigs in each treatment (n = 17,826 per treatment). Letters designate significant differences from Tukey’s HSD post-hoc testing (p < 0.05) after a one-way ANOVA and x-axis corresponds to (b). Error bars are standard error. Color represents treatment (d) Heatmap of all contigs which were differentially expressed in any treatment (n = 5287), with hierarchical clustering of treatments and contigs. Color represents expression value. Source data are provided as a Source Data file.