Fig. 1: Hx reduces Sirt2 expression in mature WM OLs.

a Representative western blots for Sirt2 and Sirt1 proteins in subcortical WM of Nx and Hx animals at postnatal days P11, P18, and P45. b Quantification of western blots. Graph displays mean ± SEM values (n = 3 brains per condition). At P11: Sirt1 ns p = 0.04793, Sirt2 *p = 0.0108; at P18: Sirt1 **p = 0.0044, Sirt2 ****p < 0.0001; at P45: Sirt1 ns p = 0.2347, Sirt2 **p = 0.0033 (Student’s t test). c, f, i, l, o Coronal sections of subcortical WM stained for Sirt2⁺ c, Sirt2⁺Olig2⁺ f, Sirt2⁺NG2⁺ i, Sirt2⁺PDGFRα⁺ l, and CC1⁺Sirt2⁺ o cells in Nx and Hx mice at P18. Dotted lines delineate WM. WM, white matter. Arrows point to nuclear Sirt2⁺ staining. Scale bar = 100 µm. d, e Quantification of the total Sirt2⁺ cell density d and percentage of Sirt2⁺ cells e in WM at P18 (****p < 0.0001 for d, e, n = 4 mice per group, Student’s t test). g, h Quantification of the total Olig2⁺Sirt2⁺ cell density (****p < 0.0001, n = 4 Nx and 5 Hx mice, Student’s t test) g and percentage of Sirt2⁺ OL lineage cells (***p = 0.0003, n = 4 Nx and 5 Hx mice, Student’s t test) h in WM at P18. j, k Quantification of the total NG2⁺Sirt2⁺ cell density (ns p = 0.9543, n = 4 per group, Student’s t test) j and percentage of Sirt2 expression in NG2⁺ OPCs in WM at P18 (ns p = 0.7479, n = 4 per group, Student’s t test) k. m, n Quantification of total PDGFRα⁺Sirt2⁺ cell density (ns p = 0.7748, n = 5 Nx and 4 Hx mice, Student’s t test) m and percentage of Sirt2 expression in PDGFRα⁺ OPCs (ns p = 0.9960, n = 5 Nx and 4 Hx mice, Student’s t test) n. p, q Quantification of the total CC1⁺Sirt2⁺ cell density (***p = 0.0004, n = 4 per group, Student’s t test) p and percentage of Sirt2⁺ mature CC1⁺-expressing OLs (**p = 0.0025, n = 4 per group, Student’s t test) q in WM at P18. Graphs display mean ± SEM values. All statistical tests are two-sided. Source data are provided as a Source Data file.