Fig. 3: Sirt2 overexpression alters oligodendrogenesis in vivo.
From: Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia
a, b Experimental approach for genetic Sirt2 overexpression in PDGFRα+ OPCs or PLP+ mature OLs in combination with Hx paradigm. c, d Coronal sections of subcortical WM stained for CC1 from Sirt2STOPPDGFRαCreERT c and Sirt2STOPPLPCreERT d transgenic mice, with respective controls, after Nx and Hx. White lines delineate WM. WM, white matter. e, g Quantification of the total CC1+ cell density in WM at P18 (WT: Nx vs Hx *p = 0.0433, Sirt2STOPPDGFRαCreERT: Nx vs Hx ***p = 0.0005, WT Nx vs Sirt2STOPPDGFRαCreERT Nx ****p < 0.0001, WT Hx vs Sirt2STOPPDGFRαCreERT Hx **p = 0.0031, n = 4 WT-Nx, 3 WT-Hx, 3 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) e, and (WT: Nx vs Hx *p = 0.0491, Sirt2STOPPLPCreERT: Nx vs Hx ***p = 0.0002, WT Nx vs Sirt2STOPPLPCreERT Nx *p = 0.0176, WT Hx vs Sirt2STOPPLPCreERT Hx ns = 0.8791, n = 3 WT-Nx, 4 WT-Hx, 4 Sirt2STOP-Nx, 4 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) g. i, j Coronal sections of subcortical WM stained for Olig2 from Sirt2STOPPDGFRαCreERT i and Sirt2STOPPLPCreERT j transgenic mice, with respective controls, after Nx and Hx. Scale bar = 100 µm. k, m Quantification of the total Olig2+ cell density in WM at P18 (WT: Nx vs Hx ***p = 0.0002, Sirt2STOPPDGFRαCreERT: Nx vs Hx ***p = 0.0001, WT Nx vs Sirt2STOPPDGFRαCreERT Nx *p = 0.0263, WT Hx vs Sirt2STOPPDGFRαCreERT Hx *p = 0.0357, n = 3 WT-Nx, 4 WT-Hx, 4 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) k, and (WT: Nx vs Hx *p = 0.0219, Sirt2STOPPLPCreERT: Nx vs Hx ****p < 0.0001, WT Nx vs Sirt2STOPPLPCreERT Nx ****p < 0.0001, WT Hx vs Sirt2STOPPLPCreERT Hx ns = 0.8177, n = 3 WT-Nx, 3 WT-Hx, 4 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) m. f, h, l, n Quantification of the percent of reduction of CC1+ and Olig2+ cells after Hx in each transgenic mouse strain. No changes were found in percent of CC1+ and Olig2+ cell reduction in WM of Sirt2STOPPDGFRαCreERT or Sirt2STOPPLPCreERT and their WT littermates. All graphs display mean ± SEM values, except for percent reduction. All statistical tests are two-sided. Source data are provided as a Source Data file.
