Fig. 4: Sirt2+ OLs are protected from Hx only in Sirt2STOPPDGFRαCreERT mice.
From: Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia
a Color legend for different transgenic mice in Nx and Hx. b Experimental Hx paradigm. c, d Coronal sections of subcortical WM stained for CC1 and Sirt2 from Sirt2STOPPDGFRαCreERT c and Sirt2STOPPLPCreERT d transgenic mice, with respective controls, after Nx and Hx. White lines delineate WM, WM-white matter. Scale bar = 100 µm. e, g Quantification of the total CC1+Sirt2+ cell density in WM at P18 (WT: Nx vs Hx **p = 0.0037, Sirt2STOPPDGFRαCreERT: Nx vs Hx ns = 0.3774, WT Nx vs Sirt2STOPPDGFRαCreERT Nx ***p = 0.0002, WT Hx vs Sirt2STOPPDGFRαCreERT Hx ****p < 0.0001, n = 4 WT-Nx, 4 WT-Hx, 3 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) e, and (WT: Nx vs Hx *p = 0.0220, Sirt2STOPPLPCreERT: Nx vs Hx ***p = 0.0002, WT Nx vs Sirt2STOPPLPCreERT Nx *p = 0.0104, WT Hx vs Sirt2STOPPLPCreERT Hx ns = 0.5991, n = 3 WT-Nx, 4 WT-Hx, 4 Sirt2STOP-Nx, 4 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) g. i, j Coronal sections of subcortical WM stained for Olig2 and Sirt2 from Sirt2STOPPDGFRαCreERT i and Sirt2STOPPLPCreERT j transgenic mice, with respective controls, after Nx and Hx. Scale bar = 100 µm. k, m Quantification of the total Olig2+Sirt2+ cell density in WM at P18 (WT: Nx vs Hx *p = =0.0415, Sirt2STOPPDGFRαCreERT: Nx vs Hx ns = 0.3589, WT Nx vs Sirt2STOPPDGFRαCreERT Nx ***p = 0.0001, WT Hx vs Sirt2STOPPDGFRαCreERT Hx ****p < 0.0001, n = 3 WT-Nx, 4 WT-Hx, 4 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) k, and (WT: Nx vs Hx ****p < 0.001, Sirt2STOPPLPCreERT: Nx vs Hx ****p = 0.0001, WT Nx vs Sirt2STOPPLPCreERT Nx ***p = 0.0009 WT Hx vs Sirt2STOPPLPCreER Hx ns = 0.9780, n = 3 WT-Nx, 3 WT-Hx, 4 Sirt2STOP-Nx, 3 Sirt2STOP-Hx mice, ANOVA with Tukey’s multiple comparisons adjustment) m. f, h, l, n Quantification of the percent of reduction of CC1+Sirt2+ and Olig2+Sirt2+ cells after Hx in each transgenic mouse strain. Changes in the percentage of CC1+Sirt2+ and Olig2+Sirt2+ cell reduction after Hx were found only in WM of Sirt2STOPPDGFRαCreERT, but not Sirt2STOPPLPCreERT mice. All graphs display mean ± SEM values, except for percent reduction. All statistical tests are two-sided. Source data are provided as a Source Data file.
