Fig. 5: Hx increases the interaction of Sirt2 with FoxO1 and p27Kip1 in WM.
From: Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia
a Cartoon depicting the FoxO1/p27Kip1 pathway regulated by Sirt2. b Color legend for different transgenic mice in Nx and Hx. c Western blots for expression of phosphorylated Sirt2 at Ser331, p27Kip1, and FoxO1 proteins in WM lysates of Sirt2STOPPDGFRαCreERT mice and their WT littermates. d–f Quantification of protein levels of pSirt2 d, p27Kip1 e, and FoxO1 f in Nx and Hx WM of WT and Sirt2STOPPDGFRαCreERT mice (pSirt2: **p = 0.0064, *p = 0.0428, ***p = 0.0002; p27Kip1: Nx vs Hx *p = 0.0200, Hx vs Hx *p = 0.0168; FoxO1: Nx vs Hx ***p = 0.0009, Hx vs Hx ***p = 0.0004; n = 3 per group, ANOVA with Tukey’s multiple comparisons adjustment). Graphs display mean ± SEM values. g Co-immunoprecipitation of dissected WM with Sirt2 antibody followed by Western blot for p27Kip1 and FoxO1, respectively to detect Sirt2 protein interactions. Protein complexes were identified by their sizes (27KD and 78-82KD, respectively). i Western blots for acetyl lysine levels of p27Kip1 and FoxO1 proteins. h, j Quantification of co-immunoprecipitation results for Sirt2/p27Kip1 (**p = 0.0054), Sirt2/FoxO1 (***p = 0.0003) h, acetyl lysine p27Kip1 (****p < 0.0001), acetyl lysine FoxO1 (***p = 0.0005), j (n = 5 Nx and Hx brains for Sirt2/p27, 6 Nx and Hx brains for Sirt2/Foxo1, 6 Nx and Hx brains for p27 acetyl, 4 Nx and Hx brains for Foxo1 acetyl, all Student’s t tests). Graphs display mean ± SEM values. All statistical tests are two-sided. Source data are provided as a Source Data file.
