Fig. 6: Sirt2 interacts with p21Cip1 and Cdk5.
From: Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia
a Cartoon depicting cell cycle regulation by p21Cip1, Cdk4/CyclinD1, and Cdk5/p35. b Expression of p21Cip1, Cdk5, p35, Cdk4, CyclinD1, p107, and E2F4 in Nx and Hx WM of WT-PDGFRαCreERT and Sirt2STOPPDGFRαCreERT. c Color legend for different transgenic mice in Nx and Hx. d–j Quantification of protein levels of p21Cip1 (WT Nx vs Hx ***p = 0.0001, WT Hx vs Sirt2STOP Nx ***p = 0.0001, WT Hx vs Sirt2STOP Hx ****p < 0.0001) d, Cdk5 (WT Nx vs Hx **p = 0.0076, WT Hx vs Sirt2STOP Nx **p = 0.0068, WT Hx vs Sirt2STOP Hx **p = 0.0068) e, p35 (WT Nx vs Hx *p = 0.0193, WT Hx vs Sirt2STOP Nx *p = 0.0106, WT Hx vs Sirt2STOP Hx **p = 0.0077) f, Cdk4 (*p = 0.0292) g, CyclinD1 (*p = 0.0243) h, p107 (***p = 0.0005) i, and E2F4 (***p = 0.0002) j in WT-PDGFRαCreERT and Sirt2STOPPDGFRαCreERT mice (n = 3 per group, all ANOVA test with Tukey’s multiple comparisons adjustment). Graphs display mean ± SEM values. k Co-immunoprecipitation of: Sirt2/p21Cip1 and acetyl-lysine p21Cip1, Cdk4/CyclinD1 and p107/E2F, Sirt2/Cdk5, acetyl-lysine Cdk5, and Cdk5/p35 complexes from Nx and Hx WM. Protein complexes were identified by their sizes (p21Cip1−21kDa, Cdk4-34kDa, Cdk5-35kDa, CyclinD1-36kDa, p35-28kDa, p107-121kDa, E2F4-62kDa, respectively) l–n Quantification of co-immunoprecipitation results for Sirt2/p21Cip1 (**p = 0.0097), acetyl lysine p21Cip1 (*p = 0.0190) l, Cdk4/cyclinD1 (*p = 0.0138), p107/E2F4 (**p = 0.023) m, Sirt2/Cdk5 (**p = 0.0046), acetyl lysine Cdk5 (*p = 0.0349), Cdk5/p35 (**p = 0.0048) n (n = 3 brains per group, all Student’s t tests). Graphs display mean ± SEM values. All statistical tests are two-sided. Source data are provided as a Source Data file.
