Fig. 4: In vitro antitumor activity evaluation of PAA4 and PAA5.

a Cell viability assay of EJ and HUVEC cells treated with PAA4 (IC50 0.8 μM for EJ cells and 2.1 μM for HUVEC cells) and PAA5 (IC50 1.0 μM for EJ cells and 2.7 μM for HUVEC cells) for 24 h. b Changes of PTGS2 mRNA in EJ cells treated by PAA4, PAA5, and PA1 for 6 h. c Fluorescence intensity of lipid peroxidation labeled by BODIPY-C11 in EJ cells. DFO (100 μM) or NAC (3.0 mM) was pretreated 2 h before the PAA4 (4.0 μM, 6 h incubation) treatment. d Rescue effect of DFO (100 μM) or NAC (3.0 mM) on the PAA4-treated (4.0 μM) EJ cell lines. DFO (100 μM) or NAC (3.0 mM) was pretreated 2 h before PAA4 (4.0 μM, 6 h incubation) treatment. Asterisks (*) denote the statistical significance: 0.001< ** P ≤ 0.01, 0.0001< *** P ≤ 0.001, **** P ≤ 0.0001, P values were performed with one-way ANOVA followed by post hoc Tukey’s test. Data were expressed as mean ± SD (n = 3 independent experiments examined over triplicates). Source data are provided as a Source Data file.