Fig. 6: In vivo antitumor activity evaluation of PAA4 and PAA5 in orthotopic bladder cancer mouse model.

a Left: schematic illustration of intravesical delivery of Au clusters. Right: timeline in treatment and bioluminescence imaging of orthotopic bladder cancer mice. b In vivo bioluminescence images of orthotopic bladder cancer mice at different days after the treatment with PBS, PAA4 and PAA5 (1.5 μM, 100 μL, 60 min). c Quantitative bioluminescence intensity of luciferase in orthotopic bladder tumor. The statistical comparison tumor growth curve was calculated on day 21. d Kaplan–Meier survival curve of EJ orthotopic bladder cancer nude mice. e Blood biochemistry data of the mice after the treatment with Saline, PAA4 and PAA5, respectively, in the aspects of major indicators of liver function including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels and important indicators of kidney function including the blood urea nitrogen (BUN) and creatinine (CRE) levels. f Immunofluorescence analyses of PTGS2 expression in EJ tumor sections after the treatment with PBS, PAA4 and PAA5. Scale bar: 40 mm. Asterisks (*) denote the statistical significance: ****P ≤ 0.0001, P values were performed with one-way ANOVA followed by post hoc Tukey’s test. Data are presented as the mean ± SD (n = 6 mice in three independent groups from a representative experiment). Source data are provided as a Source Data file.