Fig. 2: Optimization of labeling frequency.
From: Efficient DNA fluorescence labeling via base excision trapping
a Fluorescence intensity depending on the number of labeling sites in ODNs after 1000 min of reaction. b Fluorescence intensities of different numbers of CCVJ-1 in varied distances. c Fluorescence enhancement during the in situ synthesis and BETr labeling with the template ODNs with consecutive dA. d Fluorescence enhancement measured with DNAs containing one dU at varied positions from the end of the strand. [CCVJ-1] = 20 µM, [UDG] = 10 U/mL, [Templates] = 1 µM, [Primers] = 5 µM, [dATP, dCTP, dGTP] = 50 µM, [dUTP] = 100 µM, [Polymerase] = 2 U/mL. Data acquisition after 2.5 h incubation, measured in a Fluoroskan Ascent microplate reader (485/538 nm).
