Fig. 1: AMPK-α1/α2 deletion precipitates premature death. | Nature Communications

Fig. 1: AMPK-α1/α2 deletion precipitates premature death.

From: AMPK deficiency in smooth muscles causes persistent pulmonary hypertension of the new-born and premature death

Fig. 1

a Exemplar image of a section of pulmonary arterial media excised by laser microdissection. b Exemplar gels for quantitative RT-PCR amplicons for transgelin, AMPK-α1 and AMPK-α2 taken from sections of the medial layer excised from pulmonary arterial sections from AMPK-α1/α2 double knockout (KO) and AMPK-α1/α2 floxed (FLX) mice by laser microdissection. NTC negative template control (eluate from the RNA extraction for which no sample material was added). c Kaplan–Meyer curves for AMPK-α1/α2 FLX (black n = 4, 2 males and 2 females), AMPK-α1 knockouts (AMPK-α1 KO; red, n = 6, 3 males and 3 females), AMPK-α2 knockouts (AMPK-α2 KO; blue, n = 7, 2 males and 5 females) and AMPK-α1/α2 KO (orange, n = 4, 2 males and 2 females). d Retrospective analysis comparing survival rates for AMPK-α1/α2 KO males (n = 6) and females (n = 10).

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