Fig. 4: AMPK-α1/α2 deletion reduces alveolar number and precipitates thickening of alveolar walls. | Nature Communications

Fig. 4: AMPK-α1/α2 deletion reduces alveolar number and precipitates thickening of alveolar walls.

From: AMPK deficiency in smooth muscles causes persistent pulmonary hypertension of the new-born and premature death

Fig. 4

a Representative images of alveoli in lung slices from terminal samples stained for α-smooth muscle actin. b Scatter plot shows alveolar number per mm2 for terminal (n = 4 mice) and non-terminal (n = 5 mice) samples of AMPK-α1/α2 knockouts (AMPK-α1/α2 KO) vs age-matched AMPK-α1/α2 floxed controls (AMPK-α1/α2 FLX; n = 4 mice). The analysis in (b) was averaged from 2 mm2 area per mouse. c Representative images of alveoli in lung slices from terminal samples stained for α-smooth muscle actin. d Scatter plot shows alveolar wall thickness for terminal (n = 4) and non-terminal (n = 4) samples of AMPK-α1/α2 KO vs age-matched AMPK-α1/α2 FLX (n = 4). The analysis in d was averaged from 60–70 septa per mouse. Data are expressed as mean ± SEM. Statistical significance was assessed by two- sided unpaired Mann–Whitney’s test.

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