Fig. 5: SEC-SAXS analysis of NKR-P1:LLT1 shows higher-order complex formation.

Overlay of the size-exclusion chromatography profile (black line) and SAXS scattering signal (red line) for the NKR-P1:LLT1 equimolar mixture at a 15 mg/ml loading concentration. Both signals show two distinct peaks. For each collected SAXS frame, the radius of gyration was calculated using AUTORG (blue circles). For further analysis, six intervals of the SAXS data were selected and separately merged (frames 355–367, 368–378, 379–388, 389–399, 431–440, 481–491; denoted as columns with diagonal hatching). SAXS scattering curve (a), Kratky plot (b), and pair distance distribution function (c) for the intervals 389–399 and 481–491 are shown in the inset for data quality assessment of the merged data. All six merged data intervals were then individually analyzed by OLIGOMER (Supplementary Data 1 and Supplementary Fig. 5). Diffraction data from the SEC-SAXS experiment have been deposited (https://doi.org/10.17632/268ww2m4j3.1)⁸¹.