Fig. 6: Soluble LLT1 affects NKR-P1 distribution on the cell surface.

NKR-P1 stable transfectants were incubated in the presence or absence of soluble LLT1 or LLT1^SIM, and the cell surface distribution of NKR-P1 was monitored by super-resolution microscopy. a Representative brightfield (BF) and dSTORM images of full-length NKR-P1 HEK293 stable transfectants on PLL-coated slides incubated without (black) or with LLT1 (red) or LLT1^SIM (green), fixed and stained with AlexaFluor® 647-labeled anti-NKR-P1 mAb; scale bars represent 5 µm. The 10 µm² regions (red boxes in dSTORM images) are magnified and shown with corresponding clusters of fluorescent events (CoE) maps and binary maps; scale bars represent 1 µm. b–g Analysis of full-length NKR-P1 distribution relative changes induced by the presence of its LLT1 or LLT1^SIM soluble ligands: average cluster of events area (b), average cluster of events diameters (c), size distributions of cluster of events diameters overlaid with Poisson distribution functions (d), average events per cluster of events (e), density of events detected per cluster of events (f) and total density of the detected events (g). In b, c and e, f, each plotted point represents the mean value obtained from the analysis of the total inner surface of a single cell. The box plot center represents the overall mean value, the bounds of the box represent the interquartile range, and the whiskers represent ±SD. Data are from n = 45 NKR-P1⁺ control cells and n = 41 or n = 47 NKR-P1⁺ cells incubated with LLT1 or LLT1^SIM in seven or four independent experiments, respectively. One-way ANOVA with Bonferroni correction, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns. not significant. Source data are provided as a Source Data file.