Fig. 7: Inhibitory effect of soluble LLT1 on the NK cell-mediated cytotoxicity. | Nature Communications

Fig. 7: Inhibitory effect of soluble LLT1 on the NK cell-mediated cytotoxicity.

From: Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse

Fig. 7

NK cells from three different donors (blue, red, and green) and K562 target cells were incubated with PBS buffer only (negative control) or with the soluble LLT1 or LLT1SIM proteins, both in 50 and 250 µM concentrations corresponding to the 1× and 5× KD values, respectively, as analyzed by the AUC for the primary interaction mode (cf. Supplementary Fig. 4). Bar charts represent the means of live K562 cells in each condition with results of individual experiments plotted as empty circles, and the whiskers represent ±SD. When applicable, data were statistically evaluated by one-way ANOVA. Considering p < 0.05 as statistically significant, the inhibitory effect of soluble LLT1 significantly differs from LLT1SIM, which does not differ from the control condition lacking any NKR-P1 ligand. Source data are provided as a Source Data file.

Back to article page