Fig. 6: Mutational analysis of THC-binding pocket.
From: Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs
a Residues lining the THC-binding pocket and along the putative coupling pathway. b Representative TEVC recordings of GlyR WT and mutants activated by 0.1 mM glycine, and with co-application of 3.2 μM THC. Membrane potential was held at −60 mV. c Percent potentiation is plotted as (peak of THC-glycine current / peak glycine current) x 100 for WT (n = 10), S320A (n = 13) W263F (n = 9), F266A (n = 12), W267F (n = 6), P274A (n = 9), F418A (n = 8). Data are shown as mean ± s.e for (n) independent experiments. Electrophysiology experiments were performed on independent oocytes, from multiple different surgeries. Two-sided Mann–Whitney test *P = 0.0358 (S320A), **P = 0.009 (F266A), **P = 0.0075 (W267F), ***P = 0.0007 (F418A), ****P = 0.00005 (W263F), ****P = 0.00002 (P274A). Source data are provided as a Source Data file.
