Fig. 1: VHL expression in T cells is critical for the control of M. tuberculosis infection in mice.

a, b Vhl cKO and WT mice were infected via aerosol with 250 M. tuberculosis and sacrificed at indicated time points after infection. The log₁₀ CFU in the lung (a) and spleen (b) of individual mice (n WT = 10, 9, 12; n Vhl cKO = 9, 9, 10 mice at 4, 6 and 8 weeks post infection (w.p.i.) respectively) are represented. c The weight change with respect to the uninfected group mean (day 0) during infection with M. tuberculosis of Vhl cKO and WT mice (n = 11 per group) is depicted. d Representative micrographs from hematoxylin-eosin-stained paraffin lung sections from Vhl cKO and WT mice 8 w.p.i. with M. tuberculosis (bar: 400 μm). e The cumulative mortality of Vhl cKO and WT mice (n = 9 per group) after M. tuberculosis infection is depicted. f The log₁₀ CFU in the lungs and spleens of Hif1a cKO (n = 11) and WT (n = 9) mice 8 w.p.i. with M. tuberculosis are shown. g–i The representative dot plots of CD4 and CD8 (gated on T cells) in the lungs of Vhl cKO and WT mice before and 8 w.p.i. with M. tuberculosis (g), and the frequency of CD4 (h) and CD8 (i) T cells in the lungs (WT n = 4, 4, 6, 8 and Vhl cKO n = 4, 5, 6 and 8 mice at 0, 4, 6 and 8 w.p.i. respectively). j, k The frequency of CD4 and CD8 T cells in the lungs of Hif1a cKO and WT mice before (n = 4 per group) and 8 w.p.i. (n = 5 per group) are depicted. l, m The frequency of CD4 in spleens (l) and mediastinal lymph nodes (m) before and 8 w.p.i. with M. tuberculosis are shown (Vhl cKO n = 4, 7 and WT n = 4, 4 mice at 0 and 8 w.p.i., respectively). Rag2^−/− mice were administered i.v. with either 2.10⁶ Vhl cKO or WT CD4 T cells (n = 6) or left untreated 3 days after M. tuberculosis infection. The log₁₀ CFU in the lungs and spleens (n) (n = 8 animals per group) and number of CD4 T cells (o) in lungs (n = 6 mice per group) 4 w.p.i. Each symbol represents one mouse, and the data are presented as the mean ± s.e.m. The p-values were calculated using either a two-tailed unpaired t test with Welch’s correction for no homoscedasticity and FDR approach for multiple comparison (a, b, f, h–m, o), a one-way ANOVA with Welch’s correction (n), a 2-way ANOVA with Sidak’s multiple comparison test (c), or a χ² test (e). Source data are provided as a Source Data file.