Fig. 2: Reduced accumulation of mycobacteria-specific T cells in the lungs of M. tuberculosis infected Vhl cKO mice.
From: HIF-1 stabilization in T cells hampers the control of Mycobacterium tuberculosis infection
a Representative dot plots of tetramer ESAT-6-binding CD4 T cells in the lungs from Vhl cKO and WT mice 8 weeks post infection (w.p.i.) with M. tuberculosis are depicted. b The percentage of tetramer ESAT-6-binding CD4 T cells in the lungs of Vhl cKO and WT mice (WT n = 5, 5, 7 and Vhl cKO n = 5, 7, 7 mice at 4, 6 and 8 w.p.i.) are displayed. c Percentage of tetramer ESAT-6 + CD4 T cells in the lungs of Hif1a cKO (n = 5) and WT (n = 5) mice 8 w.p.i. (d, e) Dot plots (d) and the frequency of tetramer TB10.4-binding CD8 T cells (e) in the lungs from Vhl cKO (n = 5, 6) and WT mice (n = 5, 7 animals at 4 and 8 w.p.i., respectively) are depicted. f The frequency of tetramer TB10.4-binding lung Hif1a cKO (n = 5) and WT (n = 5 mice) CD8 T cells at 8 w.p.i. is depicted. g, h The frequencies of IFN-γ secreting CD4 (g) and CD8 (h) T cells from Vhl cKO (n = 9) and WT (n = 9) mice at 8 wp.i. stimulated with either ESAT-61-15 or TB10.44-11 peptides or left untreated are shown. i Dot plots showing the IFN-γ secretion by lung CD8 T cells from Vhl cKO and WT mice 8 weeks after M. tuberculosis infection. j, k The frequency of Ki-67 + , tetramer-binding and total CD4 (j) and CD8 (k) T cells from lung of Vhl cKO (n = 5) and WT mice (n = 5) 8 w.p.i. l The fraction of lung Annexin V + CD4 and CD8 T cells from Vhl cKO (n = 5) and WT (n = 6) mice at 8 weeks after M. tuberculosis infection is shown. m, n The percentage of Foxp3 + CD4 T cells in the lungs (m) and spleens (n) of Vhl cKO and WT mice at 0 and 8 w.p.i. (Vhl cKO n = 5, WT n = 4) is depicted (lungs 0 w.p.i. WT n = 4, Vhl cKO n = 4; 8 w.p.i. WT n = 5 Vhl cKO n = 8; spleens 0 w.p.i. n = 4, Vhl cKO n = 4; 8 w.p.i WT n = 4, Vhl cKO n = 5). Each symbol represents one mouse, and the data are presented as the mean ± s.e.m. The p values were calculated using a two-tailed unpaired t test with Welch’s correction and FDR adjustment for multiple comparisons. Source data are provided as a Source Data file.
