Fig. 3: Decreased expression of differentiation markers and enhanced levels of inhibitory receptors in Vhl cKO T cells during M. tuberculosis infection.

a–c The frequency of lung CD4 (a) and CD8 (b) T_CM (CD44 + CD62L + ) from Vhl cKO (n = 4, 5, 6, 7) and WT ( Vhl cKO; WT n = 4, 5, 5 and 7 mice at 0, 4, 6, and 8 weeks after M. tuberculosis infection are shown. c Dot plot of CD44 and CD62L in lung CD8 T cells from Vhl cKO and WT mice 8 weeks after infection. d The frequencies of tetramer TB10.4-binding pulmonary CD8 T_CM and T_EM cells from Vhl cKO (n = 6) and WT (n = 7) mice 8 weeks after M. tuberculosis infection are depicted. e, f The fraction of pulmonary CD4 (e) and CD8 (f) T_CM from Hif1a cKO and WT mice at 0 (n = 4 per group) and 8 (n = 5 per group) weeks after M. tuberculosis infection are depicted. g–m Dot plots (g) and frequency of KLRG1 + and PD-1 + CD44 + CD4 (h, j) and CD8 (i, l) T cells in the lungs of Vhl cKO and WT mice at 4 and 8 weeks after M. tuberculosis infection. The MFI of PD-1 in CD4 (k) and CD8 T (m) cells in the lungs of Vhl cKO and WT mice is also shown (h–m; n = 5 and 4 mice per group at 4 and 8 weeks after infection). n, o The fractions of CTLA-4 + CD4 (n) and CD8 (o) T cells in the lungs of mice before (n = 3) and 8 weeks (n = 4 mice per group) after infection are depicted. p–r Dot plots (p) and frequencies of CXCR3+ (q) and CX3CR1+ (r) CD4 and CD8 T cells in the lungs of Vhl cKO and WT mice (n = 4 per group) 7 weeks after M. tuberculosis infection. s, t The frequency of lung parenchymal (i.v. CD45.2 negative) total (s) and tetramer binding (t) CD4 and CD8 T cells in the lungs of WT and Vhl cKO mice (n = 5 per group) 8 weeks after infection with M. tuberculosis are shown. Each symbol represents one mouse, and the data are presented as the mean ± s.e.m. The p values were calculated using a two-tailed unpaired t test with Welch’s correction and FDR approach for multiple comparison. Source data are provided as a Source Data file.