Fig. 8: HIF-1 stabilization in T cells impairs the responses to immunization with BCG.
From: HIF-1 stabilization in T cells hampers the control of Mycobacterium tuberculosis infection
a–c The frequency of total (a), CD4 (b) and CD8 (c) T cell populations in lungs before and 3 weeks after i.v. immunization with BCG are depicted. d–g The percentages (d, f) and representative dot plots (e, g) of tetramer Ag85b-binding CD4 T cells (d, e) and TB10.4 tetramer-binding CD8 T cells (f, g) in the lungs of Vhl Hif1a dcKO, Vhl cKO and WT mice (n = 5 per group) after BCG immunization are shown. The frequencies of TCM (h, i), TEM (j, k) in CD4 and CD8 T cells in lungs from Vhl Hif1a dcKO, Vhl cKO and WT mice at 0 or 3 weeks after BCG immunization are depicted. l The percentage of lung CD49d + CD8 TCM before and after BCG immunization of WT and Vhl cKO mice are shown. The frequencies of KLRG1 + (m, n) and CX3CR1 + (o, p) in CD4 and CD8 T cells in lungs from Vhl Hif1a dcKO, Vhl cKO and WT mice at 0 or 3 weeks after BCG immunization are depicted. q The fraction of CTLA-4 + CD4 and CD8 T cells in the lung of BCG immunized mice are shown. Each symbol represents one mouse, and the data are presented as the mean ± SEM (a–k, m–p) before immunization n = 3 per mice per group, after immunization n = 5 per group; l before n = 4 per group, after immunization n = 5 per group; q n = 5 per group. p-values were calculated using 2-way ANOVA test, with Sidak adjustment for multiple comparisons. Source data are provided as a Source Data file.
