Fig. 3: The biodistribution and immunomodulatory effects of PIC in vivo. | Nature Communications

Fig. 3: The biodistribution and immunomodulatory effects of PIC in vivo.

From: Multifunctional nanoparticle potentiates the in situ vaccination effect of radiation therapy and enhances response to immune checkpoint blockade

Fig. 3

The cellular uptake of Cy5-PIC in different cells within the a tumors and b TDLNs after the Cy5-PIC was intratumorally injected into B78 melanoma bearing mice. (n = 5 biologically independent samples). The gating strategy is provided in Supplementary Fig. 13 in Supplementary Information. c Scheme for the study of the immunomodulation effect of PIC + RT on the B78 tumor microenvironment. d The mRNA expression of select inflammatory and anti-inflammatory genes (Ifnβ1, Mx1, Nos2, Arg1, Ifnγ, Il6, Tnfα, Il1β, Il10 and Tgfβ1) in B78 tumors at day 15 after initiation of indicated treatments. e The mRNA expression ratios of Nos2: Arg1, Tnfα: Tgfβ1 and Il1β: Il10 in tumors at day 15 after indicated treatments. PIC: 140 μg/100 μL/dose; RT: 12 Gy. (n = 5 biologically independent samples). Data in a, b and d, e are shown as mean ± SD. Statistical significance was calculated via unpaired t-test in a, b, and one-way ANOVA test in d, e. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Source data are provided in Source Data file.

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