Fig. 6: Cytoplasmic aptitude to reorganize nuclear condensates is evolutionarily conserved in insects.
From: Cytoplasmic forces functionally reorganize nuclear condensates in oocytes
a Illustrations of Drosophila melanogaster stage 9 egg chambers composed of the oocyte surrounded by a nurse and follicular cells. At stage 9, cytoplasmic random stirring occurs in controls (left), premature microtubule-based fast streaming in actin mutants (center), and stabilization in mutants with constitutively active actin nucleators (right). b Time-projections of cytoplasmic stirring viewed with autofluorescent yolk granules in Control, cappuccino, and spire mutants, or constitutively active spire overexpression mutants (GFP-spir-D); single z-slice time-projections of 25 s shown with “GFB” LUTs. c Immunoreactivity of speckles in nuclei of oocytes with Control cytoplasmic random stirring, amplified fast streaming, or stabilization; 2 μm z-projections with nucleus regions outlined in dashed white. d Cytoplasmic stirring intensity measured in Control and mutant oocytes by image correlation analyses of cytoplasmic pixel evolution; cell number, Control = 4, cappuccino RNAi = 3, spire RNAi = 5, spire−/− = 3, GFP-spir-D = 2; error bars represent mean ± s.e.m. e Quantifications of nuclear speckle number and surface in control and mutant oocytes; cell number, Control = 8, cappuccino RNAi = 9, spire RNAi = 11, spire−/− = 7, GFP-spir-D = 10; Condensate number, Control = 236, cappuccino RNAi = 62, spire RNAi = 55, spire−/− = 74, GFP-spir-D = 437; violin plots with median ± quartiles; P values derived from two-tailed Mann–Whitney U-tests or Kruskal–Wallis tests, ns not significant, P > 0.576, *P = 0.0121, **P = 0.0037, ***P < 0.001, ****P < 0.0001. Scale bars, 20 μm. Source data are provided as a Source Data file.
