Fig. 4: Illustration for working principle of FAST-POCT platform.

a RNA adsorption step, the sample is introduced into the M inlet and injected into the mixing chamber together with the pre-stored microbeads solution. After mixing and beads extraction, the reagent is dispensed to the waste chamber. b, c Washing step, different pre-stored washing reagents are injected into the mixing chamber and after mixing and beads extraction, the reagents are transferred to the waste chamber. d Elution step, the elution reagent is injected, and after mixing and beads extraction, the reagent is transferred to the PCR reaction chamber. The curves show the working process and the related parameters in different steps. The pressure is the applied pressure through various chambers. The volume is the volume of the liquid in the mixing chamber. All the scale bars are 1 cm. Source data are provided as a Source Data file.