Fig. 4: The binding of peptide P1 alters the conformational ensemble of WT αSyn.
From: Single residue modulators of amyloid formation in the N-terminal P1-region of α-synuclein
HN-CSPs of WT αSyn upon the addition of a a 5- or 10-fold molar excess of peptide-P1, b a 10-fold molar excess of peptide P1-SG and c NMR PREs of 15N-αSyn upon the addition of equimolar MTSL-labelled peptide-P1. The black line represents the median value over a rolling window of five residues. d Difference in chemical shifts of WT αSyn at pH 7.5 and 4.5. All experiments were performed in 20 mM Tris-HCl buffer, pH 7.5, 200 mM NaCl, 15 °C. The N-terminal region is shaded light blue, NAC is in purple, and the C-terminal region is in red. The P1 and P2 regions are shown in darker blue. Source data are provided as a Source Data file.
