Fig. 5: Alanine scan to identify residues in P1 region important for amyloid formation.
From: Single residue modulators of amyloid formation in the N-terminal P1-region of α-synuclein
a Schematic showing the sequence of the P1 region of αSyn. b Amyloid kinetics of the seven Ala variants in the P1 region of αSyn determined using 100 μM αSyn at 37 °C, 20 mM Tris-HCl, 200 mM NaCl, pH 7.5, 600 rpm, coloured as in a. Data for WT αSyn and ΔP1 are shown for comparison. Inserted are representative TEM images taken at the end point of one biological replicate (n = 2). Scale bar = 200 nm. Further images for Y39A and S42A are shown in Supplemental Fig. 5. c AFM images of WT αSyn (top), Y39A (middle) and S42A (bottom) at the endpoint (110 h) of the fibril growth experiment. The inset shows an expanded scale (scale bar = 50 nm). d Height and e length/height distributions of the AFM samples (WT = 232; Y39A = 2355; S42A = 898 counts). Fibrillar species are illustrated in blue, monomers and oligomers are coloured in orange. Note that monomers/oligomers were not detected for WT αSyn. % pellet and t50 values for the aggregation assays are shown in Supplementary Table 1c. Source data are provided as a Source Data file.
