Fig. 2: Single-cell landscape of MF-DC cells in Lamina Propria of human small intestine in Ctrl, CeD and GFD samples. | Nature Communications

Fig. 2: Single-cell landscape of MF-DC cells in Lamina Propria of human small intestine in Ctrl, CeD and GFD samples.

From: Single cell transcriptomic analysis of the immune cell compartment in the human small intestine and in Celiac disease

Fig. 2

a UMAP clustering plot of CD45+ Macrophage/Dendritic (MF-DC) cells in Lamina Propria (LP) of Ctrl (control subjects), CeD (Celiac disease patients) and GFD (Celiac patients on gluten-free diet) samples with side scatter plots of canonical gene expressions (MF Macrophage, Inf-MF Inflammatory Macrophage, cDC classical Dendritic cell). b Projection of Ctrl, CeD and GFD cells on the UMAP plot (left scatter plot) and boxplots (right) showing the percentage of the MF-DC single cells across the UMAP clusters from Ctrl, CeD and GFD derived cells (center line, median; box limits, upper and lower quartiles; whiskers, minimum and maximum values; points, outliers, 1.5x interquartile range; two-sided t-test p value < = 0.05 (MFs: Ctrl vs CeD = 3.54 × 10−8, CeD vs GFD = 6.32 × 10−7; Inf-MF: Ctrl vs CeD = 1.46 × 10−7, CeD vs GFD = 9.15 × 10−7); n = 10 randomized cell subsampling). c Heatmap of the differentially expressed genes between the myeloid UMAP clusters. d Scatter plots depicting distribution of the cells between Ctrl and CeD samples together with expression pattern of selected MF genes projected on the UMAP. e Barplots of top biological processes from gene ontology analysis on differentially expressed genes in Inf-MF cluster (Benjamini FDR < 0.009).

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