Fig. 2: Analysis of the binding of ISG65 to different complement C3b fragments. | Nature Communications

Fig. 2: Analysis of the binding of ISG65 to different complement C3b fragments.

From: Invariant surface glycoprotein 65 of Trypanosoma brucei is a complement C3 receptor

Fig. 2

The lower panel shows a simplified schematic of the deposition of complement C3 on the surface of a pathogen, using surface representations of C3 (PDB ID: 2A73)30, C3b (PDB ID: 2I07)9, C3c (PDB ID: 2A74)30 and C3d (PDB ID: 1C3D)51. This is coloured with the α-chain in dark grey, the β-chain in light grey, the TED domain (which becomes C3d) in orange and the domain which is removed to become C3a in red. The upper panels show surface plasmon resonance traces for the binding of C3b, C3c and C3d to immobilised ISG65. These represent two-fold dilution series from an upper concentration of 0.5 μM for C3b and C3c, and 4 μM for C3d, and are representative of three repeats.

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