Fig. 3: Layer-specific radial direction selectivity depends on bipolar cell surround.

a Center-surround properties of BC clusters from Fig. 2 plotted against IPL depth (top) or rDS preference of modeled responses (bottom). Black and gray points represent On and Off-type BCs, respectively; gray shading marks approx. ChAT bands. ρ indicates the Spearman correlation coefficient, with p-values listed (two-sided test). b Top: density plot of BC anatomical stratification as reported earlier^18,19,30. Left: density plot of BC cluster stratification of ROIs from each cluster in Fig. 2. Colors chosen by likely matches with anatomical stratification. Gray shading marks approx. ChAT bands. Middle: correlation between BC clusters and anatomical types based on their stratification in the IPL. Right: cluster assignment and likely BC type mapped onto pixels from two example imaging fields. c Left: density plot from (b) color coded by the latency between peak of surround and center responses. Middle: cluster latency assigned to squares with greater than 0.7 correlation based on IPL stratification in (b). Right: cluster latency of surround vs. center mapped onto ROIs from two imaging fields (same fields as (b)). d Same as (c), but with all panels color coded by the cluster surround strength relative to center strength. e Same as (c), but with all panels color coded by the cluster rDS measured from RF convolution with 1000 μm/s velocity motion stimulus (Fig. 2j).