Fig. 6: The entry domain is stabilized by a conserved disulfide that promotes translocation.
From: Proteolytic processing induces a conformational switch required for antibacterial toxin delivery
a CD spectra of wild-type and Cys-free ∆VENN entry domains. b 1H-15N HSQC NMR spectra of wild-type and Cys-free ∆VENN entry domains. c Chemical denaturation of wild-type and Cys-free ∆VENN entry domains. d In vitro nuclease activity. E. coli total RNA was treated with CdiA-CTEC3006 proteins in the absence and presence of purified CdiIEC3006 immunity protein (CdiI-His6). e Purified CdiA-CTEC3006 was incubated with polymyxin B (PMB) treated E. coli cells. Total RNA was isolated for Northern blot analysis using a probe to tRNAIle. The experiment in panel e was repeated independently three times with similar results. The experiments in panels a and c were repeated independently twice with similar results. The experiments in panels b and d were performed once. Source data are provided as a Source Data file.
