Fig. 7: CdiA-CTEC3006 translocation during cell-mediated delivery.
From: Proteolytic processing induces a conformational switch required for antibacterial toxin delivery
a Competition co-cultures. Target bacteria were mixed at a 1:1 ratio with inhibitor cells that deploy the indicated CdiA-CTEC3006 variants. Viable target cells were enumerated as colony-forming units (cfu) per mL every 15 min. Presented data were the average ± SEM for three independent experiments. b In vivo nuclease activity. Total RNA was isolated from the co-cultures in panel a for Northern blot analysis using a probe to tRNAIle. c CdiA-CTEC3006 processing. Urea-soluble protein was isolated from the co-cultures in panel a for immunoblot analysis using polyclonal antisera to the N-terminal TPS transport domain of CdiA. Full-length, ∆CdiA-CT, and truncated CdiA proteins are indicated. The experiment in panel a was repeated independently three times with similar results. The experiments in panels b, c were performed independently twice with similar results. For panels e, f, denaturation of CdiA-CTs was repeated independently three times with similar results and tRNase domain denaturation was performed once. Source data are provided as a Source Data file.
