Fig. 3: Obesity-induced adipose inflammation is attenuated by lactate depletion in adipocytes. | Nature Communications

Fig. 3: Obesity-induced adipose inflammation is attenuated by lactate depletion in adipocytes.

From: Adipocyte-derived lactate is a signalling metabolite that potentiates adipose macrophage inflammation via targeting PHD2

Fig. 3

Wildtype (WT) and adipocyte-specific Ldha knockout (AKO) mice were fed with standard chow (STC) or high fat diet (HFD) for 4 months before epididymal white adipose tissue (eWAT) was isolated for analysis. a HE staining of eWAT; scale bar, 100 μm. b Immunofluorescence staining of iNOS and F4/80 in eWAT; scale bar, 100 μm. c–f SVF in eWAT was subjected to flowcytometry analysis for macrophage subtypes. STC-WT: n = 5 (c–f); STC-AKO: n = 4 (c–f); HFD-WT: n = 12 (d) or n = 11(e) or n = 10 (f); HFD-AKO: n = 7 (c–f) biologically independent animals. g–j mRNA expression of g Il-1β (STC-WT: n = 5; STC-AKO: n = 4; HFD-WT: n = 6; HFD-AKO: n = 6 biologically independent animals), h Cd11c (STC-WT: n = 4; STC-AKO: n = 4; HFD-WT: n = 7; HFD-AKO: n = 6 biologically independent animals), i Tnf (STC-WT: n = 4; STC-AKO: n = 4; HFD-WT: n = 9; HFD-AKO: n = 6 biologically independent animals) and j Ccl12 (STC-WT: n = 4; STC-AKO: n = 4; HFD-WT: n = 8; HFD-AKO: n = 7 biologically independent samples) in eWAT. k–l Serum levels of k IL-1β and l MCP1. n = 4 biologically independent animals. Data represent mean ± SEM; Significance was calculated by two-way ANOVA with post hoc Bonferroni correction (d-i).

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