Fig. 3: Mutants with defective in either perinuclear or cytoplasmic P granules exhibit defects in gene silencing.

a Fluorescent micrographs show the localization of PRG-1 and WAGO-4 in the indicated strains in the adult germline (left) and in the four-cell embryo (right). Bar, 10 micrometers. Quantification of each genotype’s effect on granule properties is provided in Supplementary Fig. 2a. b GFP expression in the piRNA reporter and the ability to form perinuclear or cytoplasmic PRG-1 granules in the indicated strains.