Fig. 5: Pentamer-destabilizing mutations impair G5C selective translation.
From: Structural basis for Gemin5 decamer-mediated mRNA binding
Protein expression in HEK293 cells was monitored by WB using anti-Xpress for G5C proteins, tubulin was used as loading control. Diagrams of the luciferase reporter mRNAs used in the assay, a luc-SL1 and b IRES-luc. Luciferase activity was measured in cell lysates expressing the reporter mRNA, a luc-SL1 or b IRES-luc, co-transfected with Xpress-G5C-WT or the indicated mutants. Luciferase values are normalized to cells expressing Xpress-G5C-WT. Values represent the mean ± SD obtained in three independent assays using two-tailed paired Student t-tests. Comparison for the same: **p < 0.01, *p < 0.05; ns, not significant. Specific p values are 0.007124, 0.001610, 0.104045 in the indicated order for (a) luc-SL1 translation, and 0.009374, 0.022961, 0.065317 for (b) IRES-luc translation. c, d Values represent the mean ± SD obtained in three independent assays using two-tailed paired Student t-tests. Comparison is for the same: ns, not significant. The specific p values are 0.389685, 0.611948, 0.171729 for (c), and 0.739000, 0.059173, 0.955913 for (d).
