Fig. 5: Interplay of PINOID/WAG activity and WAV3/WAVH genes in PIN2 polarity control.
From: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
PIN2 localization in 4 DAG wild type (w.t.; a, f; 359 cells/9 roots), wav triple (wtrp; b, f; 265 cells/11 roots), 35S::PID (c, f; 317 cells/15 roots) and wav triple 35S::PID (d–f; 429 cells/14 roots) root meristem epidermis cells. Arrowheads indicated polarity of PIN2 localization. g–k PIN2 localization in cortex (‘c.’) and epidermis (‘ep.’) root meristem cells of eir1-4 PIN2::PIN2:Dendra (eir1-4 PIN2:Den; g, k; 243 cortex cells/212 epidermis cells/14 roots), eir1-4 PIN2::pin2S1,2,3D:Dendra (eir1-4 pin2S-D:Den; h, k; 326 cortex cells/372 epidermis cells/19 roots), eir1-4 wav triple PIN2::PIN2:Dendra (eir1-4 wtrp PIN2:Den; i, k; 193 cortex cells/210 epidermis cells/17 roots) and wav triple eir1-4 PIN2::pin2S1,2,3D:Dendra (eir1-4 wtrp pin2S-D:Den; j,k; 205 cortex cells/214 epidermis cells/16 roots) at 5 DAG. Arrowheads indicated polarity of PIN2 localization. Scale bars: a–e, g–j = 20 μm. Circles represent data sets from individual root meristems; boxes: first and third quartiles; center line: median; ‘x’: mean value. One-way ANOVA with post hoc Tukey HSD was performed; *: p < 0.05; ***: p < 0.001; a–e: p > 0.05. Source data are provided as Source Data file.
