Fig. 7: Subcellular localization of WAV3/WAVH/SOR1 reporter proteins.
From: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
a Root meristem stele cells expressing WAVH1::WAVH1:Ven at 5 DAG. Root meristem epidermis cells of 5 DAG seedlings expressing WAV3::Ven:WAV3 (b); WAVH2::WAVH2:Ven (c); XVE»Ven:WAV3 in presence of 2 μM estradiol (d). Closed arrowheads indicate reporter localization proximal to the PM, open arrowheads indicate intracellular reporter signals. e Subcellular localization of GFP:SOR1 in root meristem epidermis cells of 5 DAG wav triple 35S::GFP:SOR1 seedlings (arrowheads). f PIN2 immunolocalization in root meristem epidermis cells of 5 DAG wav triple 35S::GFP:SOR1 seedlings (arrowheads). g Subcellular localization of Ven:WAV3 in a dividing root meristem cell of 5 DAG wav triple XVE»Ven:WAV3 (left, arrowhead indicates cell plate). FM4-64 co-staining was employed to highlight the emerging cell plate (right). h Localization of PIN2:mCherry and Ven:WAV3 in root meristem epidermis cells of 5 DAG PIN2::PIN2:mCherry XVE»Ven:WAV3 seedlings grown in presence of 2 μM estradiol. Closed arrowheads indicate PIN2:mCherry polarity at the PM, whilst open arrowheads indicate polarity of Ven:WAV3. i Localization of PIN2:mCherry and Ven:WAV3 in a cytokinetic root meristem epidermis cells of 5 DAG PIN2::PIN2:mCherry XVE»Ven:WAV3 grown in presence of 2 μM estradiol. Closed and open arrowheads indicate cell plate localization of PIN2:mCherry and Ven:WAV3, respectively. Three independent experiments were performed for (a–i) with similar results. Scale bars: a–d, f = 10 μm; e = 20 μm; h, i = 5 μm.
