Fig. 3: Expression of CASZ1 and RXFP2 in the adrenal cortex and APA.
From: Identification of risk loci for primary aldosteronism in genome-wide association studies
a CASZ1 and RXFP2 mRNA localization in the adrenal cortex of an adrenal with APA carrying a somatic KCNJ5 mutation analyzed by RNAscope. Red dots represent positive staining. Images are representative of results obtained in adrenals from four patients. C capsule, ZG zona glomerulosa, ZF zona fasciculata. b Uniform Manifold Approximation and Projection (UMAP) diagram from snRNA-seq from 5 pieces of adrenal cortex adjacent to APA from two adrenals; cells are colored by 1–20 cell-type clusters. c UMAP plots showing the expression of CASZ1, RXFP2, and CYPP11B2 within the different clusters. d CASZ1 mRNA localization in cells expressing CYP11B2 in the adrenal cortex of an adrenal with APA (patient 1 analyzed by snRNAseq) carrying a somatic KCNJ5 mutation. CASZ1 mRNA expression was analyzed by RNAscope; CYP11B2 protein expression was analyzed by immunohistochemistry. For each sample, one experiment was performed.
