Table 3 Steroid profiles in stably transfected H295R_S2 cells expressing RXRP2 and CASZ1

From: Identification of risk loci for primary aldosteronism in genome-wide association studies

Characteristics

Ctrl+AngII

RXFP2

RXFP2 + AngII

p value

CASZ1

CASZ1 + AngII

p value

Pregnenolone

0.90 (0.69,1.42)

0.50* (0.44,0.62)

0.36*** (0.29,0.48)

<0.0001

0.57** (0.44,0.75)

0.55** (0.34,0.65)

<0.0001

Progesterone

0.48**** (0.44,0.50)

1.23 (0.92,1.55)

0.36 (0.28,0.43)

<0.0001

1.20 (0.77,1.57)

0.53 (0.31,0.76)

<0.0001

11-deoxycorticosterone (pmol/L)

0.35**** (0.32,0.37)

0.45**** (0.37,0.53)

0.30 (0.28,0.33)

<0.0001

0.19*** (0.14,0.25)

0.17** (0.11,0.22)

<0.0001

18-hydroxy-11-deoxycorticosterone (pmol/L)

2.73*** (2.49,2.94)

0.27** (0.12,0.40)

0.41*** (0.39,0.42)

<0.0001

0.06* (0.05,0.08)

0.07**** (0.04,0.10)

<0.0001

Corticosterone (nmol/L)

4.16**** (3.64,4.71)

0.24*** (0.22,0.28)

0.97**** (0.96,1.02)

<0.0001

0.03**** (0.03,0.04)

0.19**** (0.18,0.20)

<0.0001

18-hydroxycorticosterone (pmol/L)

3.32*** (3.13,3.65)

0.51 (0.48,0.55)

1.40 (1.22,1.61)

<0.0001

0.18** (0.14,0.22)

0.42** (0.39,0.45)

<0.0001

Aldosterone (pmol/L)

4.86**** (4.53,5.00)

0.34**** (0.27,0.45)

1.57**** (1.18,2.02)

<0.0001

0.03** (0.03,0.04)

0.22** (0.18,0.28)

<0.0001

17-hydroxyprogesterone (nmol/L)

0.24**** (0.23,0.25)

1.97 (1.46,2.32)

0.35 (0.28,0.42)

<0.0001

1.56 (1.04,2.17)

0.56 (0.36,0.78)

<0.0001

11-deoxycortisol (nmol/L)

0.32*** (0.21,0.41)

1.32 (1.05,1.51)

0.72 (0.70,0.78)

<0.0001

0.78 (0.67,0.88)

0.55 (0.45,0.62)

<0.0001

Cortisol (nmol/L)

3.08**** (2.94,3.15)

0.92 (0.90,0.97)

2.24**** (2.08,2.28)

<0.0001

0.570 (0.40,0.78)

1.67 (1.44,1.94)

<0.0001

21-deoxycortisol (pmol/L)

0.70** (0.58,0.89)

2.02* (1.66,2.85)

1.49* (0.84,1.72)

0.0001

1.31 (0.38,2.66)

1.05 (0.55,1.78)

0.4796

Delta-4-androstenedione (nmol/L)

0.18**** (0.14,0.21)

0.984* (0.84,1.03)

0.22 (0.22,0.24)

<0.0001

0.69**** (0.59,0.82)

0.30** (0.23,0.37)

<0.0001

  1. Results are expressed as fold induction over mock-transfected untreated cells (Ctrl set as 1 not shown in the table) and represent median and interquartile range, compared with ANOVA followed by Sidak’s or Kruskal-Wallis followed by Dunn’s multiple comparison test. Cells overexpressing RXFP2 or CASZ1 were analyzed separately. Comparison between Ctrl vs Ctrl+Ang was performed with unpaired t-test or Mann-Whitney test to control for response of cells to AngII.
  2. Ctrl Control, AngII angiotensin II.
  3. Ctrl+AngII compared to Ctrl: ****p ≤ 0.0001; 18-hydroxy-11-deoxycorticosterone p = 0.0002; 18-hydroxycorticosterone p = 0.0002; 11-deoxycortisol p = 0.0002; 21-deoxycortisol p = 0.0082.
  4. RXFP2 vs Ctrl: ****p ≤ 0.0001; Pregnenolone p = 0.0199; 18-hydroxy-11-deoxycorticosterone p = 0.0039; Corticosterone p = 0.0003; 21-deoxycortisol p = 0.0153; Delta-4-androstenedione p = 0.0442.
  5. RXFP2 + AngII vs Ctrl+AngII: ****p ≤ 0.0001; Pregnenolone p = 0.0003;18-hydroxy-11-deoxycorticosterone p = 0.0004; 21-deoxycortisol p = 0.0227.
  6. CASZ1 vs Ctrl: ****p ≤ 0.0001; Pregnenolone p = 0.0083; 11-deoxycorticosterone p = 0.0002; 18-hydroxy-11-deoxycorticosterone p = 0.0162; 18-hydroxycorticosterone p = 0.0013; Aldosterone p = 0.0013.
  7. CASZ1 + AngII vs Ctrl+AngII: ****p ≤ 0.0001; Pregnenolone p = 0.0057; 11-deoxycorticosterone p = 0.0056; 18-hydroxycorticosterone p = 0.0013; Aldosterone p = 0.0013; Delta-4-androstenedione p = 0.0063.
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