Fig. 2: The 129-Hfe tm.1.1Nca mouse model displays increased iron accumulation.
From: In vivo adenine base editing reverts C282Y and improves iron metabolism in hemochromatosis mice
A Chromatogram of the Hfe locus obtained by Sanger sequencing of DNA of 129S6/SvEvTac or 129-Hfe tm.1.1Nca mice. The underlined nucleotides present the disease-causing mutation giving rise to C282Y. B Depiction of hepatic iron accumulation using Prussian Blue staining of liver sections obtained from wt or 129-Hfe tm.1.1Nca mice. Densitometric quantification was performed using ImageJ. Statistical significance was determined using a two-tailed unpaired t test. p value = 0.0043. Data are presented as mean values +/− SEM. Wt (129S6/SvEvTac) 0.04088 ± 0.0046, n = 12; Mut (129-Hfe tm.1.1Nca) 0.3102 ± 0.08446, n = 12. The bars in the scatter dot plot depict the mean with the SD. Male mice are represented in circles, female mice in triangles. C Transferrin saturation analysed from serum. Statistical significance was determined using a two-tailed unpaired t test. p value = 0.0003. Data are presented as mean values +/− SEM. WT (129S6/SvEvTac) 34.04 ± 2.639, n = 5; Mut (129-Hfe tm.1.1Nca) 75.00 ± 6.116, n = 5. The bars in the scatter dot plot depict the mean with the SD. Male mice are represented in circles, female mice in triangles. D UIBC analysed from serum. Statistical significance was determined using a two-tailed unpaired t test. p value = 0.0002. Data are presented as mean values +/− SEM. WT (129S6/SvEvTac) 54.89 ± 3.054, n = 5; Mut (129-Hfe tm.1.1Nca) 18.77 ± 4.742, n = 6. The bars in the scatter dot plot depict the mean with the SD. Male mice are represented in circles, female mice in triangles. E Measurement of non-heme iron levels in the liver tissue Statistical significance was determined using a two-tailed unpaired t test. p value = 0.0004. Data are presented as mean values +/− SEM. WT (129S6/SvEvTac) 0.8636 ± 0.06055, n = 8; Mut (129-Hfe tm.1.1Nca) 3.093 ± 0.4745, n = 8. The bars in the scatter dot plot depict the mean with the SD. Male mice are represented in circles, female mice in triangles and F hepcidin levels in serum of WT and 129-Hfetm.1.1Nca mice were compared. Both parameters show a significant difference between the two groups. Statistical significance was determined using a two-tailed unpaired t test. p value = 0.0183. Data are presented as mean values +/− SEM. WT (129S6/SvEvTac) 433.3 ± 37.13, n = 5; Mut (129-Hfe tm.1.1Nca) 272.8 ± 35.60, n = 4. The bars in the scatter dot plot depict the mean with the SD. Male mice are represented in circles, female mice in triangles. Source data are provided as a Source Data file.
