Fig. 6: The stability of SUMOylation-deficient MRE11 is improved by fusion with a poly-SUMO2 chain. | Nature Communications

Fig. 6: The stability of SUMOylation-deficient MRE11 is improved by fusion with a poly-SUMO2 chain.

From: Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin

Fig. 6

a Schematic of the 4KR-MRE11 protein with a 3×SUMO2 chain fused to the C-terminus. Fusion protein expression was detected by immunoblotting. b The ubiquitylation of 4KR was inhibited by fusion with the 3×SUMO2 chain. c SUMOylation-deficient MRE11 fused with poly-SUMO2 facilitated RPA2 phosphorylation and ATR-CHK1 pathway activation after DNA damage. shMRE11 HeLa cells reconstituted with SFB-WT, SFB-4KR, and SFB-4KR-poly-SUMO2 as indicated were treated with 1 μM CPT for 1 h or not. d Cell viability was determined by CCK-8 assay after treatment with 0.5 μM CPT or 1 μM cisplatin for the indicated times (means ± SEM, n = 3 independent experiments).

Back to article page