Fig. 1: In vivo therapeutic efficacy of 12H5 in mice and in vitro neutralization and binding activity of C12H5. | Nature Communications

Fig. 1: In vivo therapeutic efficacy of 12H5 in mice and in vitro neutralization and binding activity of C12H5.

From: Identification of a cross-neutralizing antibody that targets the receptor binding site of H1N1 and H5N1 influenza viruses

Fig. 1

a–d Therapeutic efficacy of 12H5 against lethal challenge of MA-CA7/2009 (a, b), and MA-QH/2005 (c, d). The body weight (a, c) and survival curves (b, d) of BALB/c mice (n = 5 per group) treated with 12H5 or PBS 24 h after lethal challenge are shown. The body weight curves reflect the weights of surviving mice; data pertaining to the mice that died after challenge were omitted from the body weight data. Data in a, c are presented as mean values ± SD. For survival data, the log-rank test was used to evaluate significance, compared to control PBS-treated group (*P < 0.05, ****P < 0.0001). e Binding reactivity of C12H5 to purified HA proteins from H1N1 and H5N1 strains. EC50 values above 104 ng/mL were recorded as unfavorable. Full strain designations are detailed in Supplementary Table 2. f Binding affinity of C12H5 to HAs of three strains. The kinetic constants represent a representative experiment from two independent experiments. Data are presented as mean values. g 50% inhibitory concentrations [IC50 (µg/mL)] were determined by hemagglutinin inhibitory assay (HAI) against the representative stains from H1N1 and H5N1. Three independent experiments were used to calculate the average values. Values below 50 µg/mL are colored as follows: dark red, extreme reactivity; red, strong reactivity; orange, moderate-high reactivity, yellow, moderate-low reactivity; light green, weak reactivity; green, very weak reactivity; >50, negative reactivity. C12G6, the negative control antibody; FL/2006, control virus. h In vitro microneutralization (IC50) values of C12H5 and the indicated antibodies against the 15 representative strains. Data represent the average values from three independent experiments and are marked with a single symbol. Data are presented as mean values +/– SEM. Group comparisons in h were made by Friedman test ANOVA with Dunn’s multiple comparisons test. *P < 0.05 and ****P < 0.0001 were considered significant. Source data are provided as a Source Data file.

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