Fig. 1: JAK2-V617F correlated with increased IL-1 expression in MPN patients. | Nature Communications

Fig. 1: JAK2-V617F correlated with increased IL-1 expression in MPN patients.

From: Inhibition of interleukin-1β reduces myelofibrosis and osteosclerosis in mice with JAK2-V617F driven myeloproliferative neoplasm

Fig. 1

a Upper panel: Serum IL-1β (pg/ml) in normal controls (NC; n = 20) and MPN patients (n = 120); ET (n = 42), PV (n = 44), PMF (n = 34). Correlation (r) between % JAK2-V617F in granulocytes and log transformed serum IL-1β in MPN patients. Limit of detection is shown by dashed green line at y = 0.01 pg/ml. Lower panel: IL-1β mRNA expression relative to β-actin in granulocytes of NC (n = 12) and MPN patients (n = 46); ET (n = 11), PV (n = 18), PMF (n = 17). Correlation between log transformed IL1B mRNA expression and % JAK2-V617F. b Upper panel: Serum IL-1RA (pg/ml) in NC (n = 20) and MPN patients (n = 120); ET (n = 42), PV (n = 44), PMF (n = 34). Correlation (r) between % JAK2-V617F and log transformed serum IL-1RA. Lower panel: IL1RN (IL-1RA) mRNA expression relative to β-actin in NC and MPN patients. Correlation between log transformed IL1RN mRNA expression and % JAK2-V617F. Two-tailed unpaired non-parametric Mann–Whitney t-test was performed in a and b. c Representative histogram showing the expression of interleukin 1 receptor type 1 (IL-1R1) in peripheral blood hematopoietic stem cells (HSCs) from isotype control, NC (n = 5), ET (n = 6), PV (n = 5), and PMF (n = 7). Bar graph showing the percentages of IL-1R1+ HSC), common myeloid progenitors (CMP), granulocyte macrophage progenitor (GMP), megakaryocyte erythroid progenitor (MEP) and megakaryocyte progenitor (MkP). Graph showing correlation (r) between % JAK2-V617F and percentages of IL-1R1 + HSCs. d Representative histogram showing the expression of interleukin 1 receptor accessory protein (IL-1RAcP) in peripheral blood HSC from NC and MPN patients. Bar graph showing the percentages of IL1RAcP+ HSC, CMP, GMP, MEP, and MkP in NC (n = 5), ET (n = 6), PV (n = 5), and PMF (n = 7). Correlation (r) between % JAK2-V617F and percentages of IL-1RAcP+ HSPCs. Two-tailed unpaired t-test was performed for statistical comparisons in c and d. Spearman correlation (r) and two-tailed t-test was performed for correlation analysis in a–d. All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. See also Supplementary Figs. 1–3. Source data and exact p values are provided as a Source Data file.

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