Fig. 3: Genetic deletion of IL-1β in a JAK2-V617F MPN mouse model. | Nature Communications

Fig. 3: Genetic deletion of IL-1β in a JAK2-V617F MPN mouse model.

From: Inhibition of interleukin-1β reduces myelofibrosis and osteosclerosis in mice with JAK2-V617F driven myeloproliferative neoplasm

Fig. 3

a Wildtype (WT; n = 9), IL-1β knock-out (IL-1β−/−; n = 11), Scl;Cre;V617F (VF; n = 18) and Scl;Cre;V617F; IL-1β knock-out (VF;IL-1β−/−; n = 13) mice were induced with tamoxifen and disease kinetics were followed for 36 weeks. Complete blood counts, grade of reticulin fibrosis at 16- and 32-weeks after tamoxifen and spleen weight at 16 weeks after tamoxifen induction are shown. Kaplan-Meier survival curve showing the percent survival of mice Grey area represents normal range. Two-way ANOVA followed by Tukey’s multiple comparison tests were used for multiple group comparisons for blood counts. Two-tailed unpaired t test was performed for spleen weight. b left panel: IL-1β protein levels in BM lavage (1 femur and 1 tibia) of WT (n = 13), VF (n = 11) and VF;IL-1β−/− (n = 18) and plasma of WT (n = 21), VF (n = 21) and VF;IL-1β−/− (n = 20) mice at 12–16 weeks after tamoxifen induction. IL-1α levels (middle panel) BM and plasma is shown (n = 4 per group). Two-tailed unpaired non-parametric Mann–Whitney t-test was performed. c Pro-Inflammatory cytokine levels (normalized to WT; dotted line) in BM lavage and plasma of WT (n = 8), VF (n = 8) and VF;IL-1β−/− (n = 4) mice at 16 weeks after tamoxifen induction. Two-tailed unpaired t-tests were performed for multiple comparisons. Grey asterisk represents the comparison between WT and VF or VF;IL-1β−/− All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. See also Supplementary Figs. 4 and 5. Source data and exact p values are provided as a Source Data file.

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