Fig. 4: Loss of IL-1β in JAK2-V617F mutant cells reduces MPN symptom burden and myelofibrosis.

a Schematic of non-competitive transplantation with 2 million BM cells from tamoxifen induced VF, WT, VF; IL-1β^−/−, or IL-1β^−/− donor mice into lethally irradiated WT recipients (n = 15 per group). Complete blood counts measured every 4 weeks until 32 weeks after transplantation are shown. Two-tailed unpaired t-tests without correction for multiple comparisons was performed. Grey area represents normal range. b Bar graph shows the spleen weight at 32 weeks after transplantation. Two-tailed unpaired t test was performed. c Representative images of bone marrow fibrosis (reticulin fibrosis) are shown at 32-weeks after transplantation. Histological grade of reticulin fibrosis in the BM at 16- and 32-weeks after transplantation is shown in the bar graph. Two-tailed unpaired t test was performed. Bar graph showing the percentage of mice with osteosclerosis in the BM. d Schematic of non-competitive transplantation with 2 million BM cells from tamoxifen induced VF, WT, VF;IL-1β−/−, or IL-1β^−/− donor mice into lethally irradiated IL-1β^−/− recipients (n = 15 per group). Complete blood counts measured every 4 weeks until 32 weeks after transplantation are shown. Two-tailed unpaired t-tests without correction for multiple comparisons was performed. Grey area represents normal range. e Bar graph shows the spleen weight at 32 weeks after transplantation. Two-tailed unpaired t test was performed. f Representative images of BM fibrosis (reticulin fibrosis) are shown at 32 weeks after transplantation. Histological grade of reticulin fibrosis in the BM at 16- and 32-weeks after transplantation is shown in the bar graph. Bar graph showing the percentage of mice with osteosclerosis in the BM. All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. See also Supplementary Figs. 6–8. Source data and exact p values are provided as a Source Data file.