Fig. 1: Generation of skeletal muscle-specific Akt1/2 knockout mice.
From: Deletion of skeletal muscle Akt1/2 causes osteosarcopenia and reduces lifespan in mice
Expression of Akt in EDL and soleus of the mAktDKO mice at the age of 8 weeks analyzed by, a RT-PCR (n = 6 mice per group, as shown by the number of data points on the graph, same as below), and b western blotting. c, d Insulin signaling in c gastrocnemius, and d soleus, at the age of 8 weeks after treatment with insulin for 10 minutes analyzed by western blotting (n = 3 or 4 mice). Values of the data are expressed as mean ± SEM. *P < 0.05, **P < 0.01. Unpaired 2-tailed t-test was used for assessment, and the exact P values are provided in Supplementary Data 2. Source data are provided as a Source Data file.
